MLH1 Knockout cell line (AGS)

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MLH1 Knockout cell line (AGS) Order

MLH1 Knockout cell line (AGS)
Catalog#
YKO-H110
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
MLH1 Knockout cell line (AGS)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
MLH1 Knockout cell line (AGS)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
MLH1
Gene ID
4292
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% F12K+10% FBS
Accession
CVCL_D8DR(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 12 (based on transcript MLH1-201)
Validation: PCR+Sanger Sequencing
MLH1 Knockout cell line (AGS) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:AGS		 STR Info (Cell bank)
Cell Line:AGS
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 912912
D13S317 12121212
D7S820 10111011
D16S539 11131113
VWA 16171617
TH01 6767
AMEL XXXX
TPOX 11121112
CSF1PO 11121112
D12S391 1920
FGA 2324
D2S1338 2022
D21S11 2929
D18S51 1313
D8S1179 1313
D3S1358 1616
D6S1043 1214
PENTAE 1316
D19S433 13.216
PENTAD 910
D1S1656 1416
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the MLH1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the AGS cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, MLH1 gene knockout MLH1 Knockout cell line (AGS) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
MLH1 Knockout cell line (AGS)
MLH1 Knockout cell line (AGS)
Catalog#
YKO-H110
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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